Insertion junction: CLIP2.042663_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042663
Chromosome:chromosome 10
Location:1070766
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g425251 (1 of 5) IPR008274 - Aldehyde oxidase/xanthine dehydrogenase, molybdopterin binding CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGAGCGGCCGAGTCCGAGGCGGCGGATGAGGACCGCGAGGAGCGAGCGG
Internal bar code:ATCGTAACAACGCGATTTCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1792
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGTCACTTGTTGCCTGC
Suggested primer 2:TAATAGGGAAAGCAGGCGCC