| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042672 |
| Chromosome: | chromosome 8 |
| Location: | 906168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361750 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAATGACTAGGCTTGGCACACAAATGCCCCCGTCCTCCGCCACAGGGG |
| Internal bar code: | CTCCGTATCGATCACCTGCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 287 |
| LEAP-Seq percent confirming: | 28.5714 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCCTGGCCCGTTATTCTA |
| Suggested primer 2: | ACCTCCGCTCTTTCTGCTTC |