| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042674 |
| Chromosome: | chromosome 2 |
| Location: | 3196698 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g800211 | (1 of 3) IPR000104//IPR020846 - Antifreeze protein, type I // Major facilitator superfamily domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTTCACCATCCTGTGGATTATGCTCACCTCGCAGCTGGGCCACTAC |
| Internal bar code: | AACTGGTTGGTCATGGATTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1255 |
| LEAP-Seq percent confirming: | 6.06061 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACCCACACCCACTAGAC |
| Suggested primer 2: | AGTATCGCGACACAGGCAAA |