Insertion junction: CLIP2.042676_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042676
Chromosome:chromosome 9
Location:4710603
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g402108 (1 of 1) PTHR13167//PTHR13167:SF25 - UNCHARACTERIZED // PIEZO, ISOFORM E; Piezo R-Ras-binding domain membrane protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTAGGCGCCGCGACCCAGCTTGGCAAGGATCTGTAAGGGCATTGGGCCA
Internal bar code:GAAGCGTTGGTGCGCTTGGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2184
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGACGTGTGACCAAACG
Suggested primer 2:TAACTCATCAACCCACCCGC