| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042676 |
| Chromosome: | chromosome 16 |
| Location: | 1302388 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651450 | (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAAGTCAGAAGGCTACGGACATGGTGCTACATGTTCCCCATCGACAT |
| Internal bar code: | GTTCGGTATTTGTGGCTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2906 |
| LEAP-Seq percent confirming: | 89.3617 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAGCTGATCAATGTGCG |
| Suggested primer 2: | TCCAGCGACACGGTATATGC |