Insertion junction: CLIP2.042703_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042703
Chromosome:chromosome 2
Location:4848002
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g109366 (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAATTGCGGCGGCAACAGGCGCAGGCGCAGGCGCTGACAGGTCAAGGC
Internal bar code:ATGATAGCAAAAGGGAATCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2523
LEAP-Seq percent confirming:56.8182
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TGAATAAACACACGCCCCGA
Suggested primer 2:GGTTTGCAACTGCTACGGTG