| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042703 |
| Chromosome: | chromosome 2 |
| Location: | 4848205 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109366 | (1 of 68) 2.1.1.43 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGCATGCCAGGCCAGGTGATGCCTCAGACGGGTGACTAGGCAGGCGAC |
| Internal bar code: | ATGATAGCAAAAGGGAATCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2693 |
| LEAP-Seq percent confirming: | 70.4225 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTGCAACTGCTACGGTG |
| Suggested primer 2: | TGAATAAACACACGCCCCGA |