Insertion junction: CLIP2.042705_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042705
Chromosome:chromosome 10
Location:5017586
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g454550 COX19 (1 of 1) K18183 - cytochrome c oxidase assembly protein subunit 19 (COX19); Mitochondrial cytochrome c oxidase assembly protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCACGACGCTGACGCGAACACATCCAATAGCCCGACCCCCGCCAAACT
Internal bar code:GAGGAATGAGAGTTCAACTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1596
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGTTGTCCCGCGATTCT
Suggested primer 2:GCATTGACGTGCATGGGTTT