Insertion junction: CLIP2.042707_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042707
Chromosome:chromosome 1
Location:6067759
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042850 (1 of 2) K17278 - membrane-associated progesterone receptor component (PGRMC1_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGACAATAGGCTGAGGTAGCAGGGACCGTTTGCGTGTAGGGTTGGTTT
Internal bar code:TCGGTCGGGTTGATGCCAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1938
LEAP-Seq percent confirming:91.8367
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GCTATCTTGCTCGCAACACG
Suggested primer 2:GAGGGGTAACAACCTGGAGC