| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042713 |
| Chromosome: | chromosome 12 |
| Location: | 5769392 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532650 | (1 of 1) PTHR21394:SF0 - MAU2 CHROMATID COHESION FACTOR HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAGCACAAGGGAAACCATACGCGCAGGACAGTGTATGCGGTGCAGG |
| Internal bar code: | TAGAATGCTCTGGAGTCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 725 |
| LEAP-Seq percent confirming: | 37.234 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGTCAAGCAATGCAGTGC |
| Suggested primer 2: | GAGTGGTAGTGGTGGTGGTG |