| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042741 |
| Chromosome: | chromosome 14 |
| Location: | 1234208 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g615950 | Soluble ABC-E class cytosolic protein; (1 of 2) K03235 - elongation factor 3 (EF3, TEF3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATGCGGCCATGCCCAAACGTTCACACGCGACAATATCAAGTTAAACC |
| Internal bar code: | ATTGGGTCGTATGTTACCACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 66 |
| LEAP-Seq percent confirming: | 5.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTCCTGCTCATCCCACT |
| Suggested primer 2: | GACCATCGCGACTCCGTTAT |