Insertion junction: CLIP2.042747_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042747
Chromosome:chromosome 6
Location:8337521
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g307400 CCR5,EEP4 (1 of 2) PTHR12121//PTHR12121:SF37 - CARBON CATABOLITE REPRESSOR PROTEIN 4 // 2',5'-PHOSPHODIESTERASE 12; Exonuclease-Endonuclease-Phosphatase superfamily protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACGAAATCTCGGTGGTGTGCTACAACCTGCTTGCCGACGTGTATGCC
Internal bar code:CCGGACGCGCTCGATCGAGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1566
LEAP-Seq percent confirming:82.5397
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TGGACACAATCTCCTGCCAC
Suggested primer 2:CCTTCATTTGGCTCCTCGGT