Insertion junction: CLIP2.042759_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042759
Chromosome:chromosome 2
Location:2112643
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089200 HLM4,SET3 (1 of 1) PTHR22884//PTHR22884:SF370 - SET DOMAIN PROTEINS // HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-9 SPECIFIC SUVH5-RELATED; Histone-lysine N-methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTTCTCCTTCTTGCCGCCGCCCTTGCCGCCCTTCCCCTTCTTGGCGC
Internal bar code:AACTAAATGATGCAGTCCAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:40.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GAAGACGGGAGGTTGGAAGG
Suggested primer 2:ACTTGTACTCCACCTTGGCG