| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042775 |
| Chromosome: | chromosome 16 |
| Location: | 7238564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677205 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGAGTAGACCAGGCCCACGTTGGACAGCGTCAGGCGGCCCAGGTTGG |
| Internal bar code: | GTTTATAAATTAAGAATTGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3320 |
| LEAP-Seq percent confirming: | 34.5238 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGGTATTTGATGCGGTG |
| Suggested primer 2: | ATGTGCTTGTGGTTTGCGAC |