| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042775 |
| Chromosome: | chromosome 16 |
| Location: | 7238566 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677205 | (1 of 4) PF00207 - Alpha-2-macroglobulin family (A2M) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCGCTGGTACTCCTGGACGCCGACACGGTGAGGGACACGCAGAGTGC |
| Internal bar code: | TTGGCATTCACTTTCGCCATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3431 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCTTGTGGTTTGCGAC |
| Suggested primer 2: | CGCTGGTATTTGATGCGGTG |