Insertion junction: CLIP2.042789_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042789
Chromosome:chromosome 9
Location:409070
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre18.g749697 FUT11 a-1%252C3-fucosyltransferase; (1 of 1) PTHR11929:SF131 - ALPHA-(1,3)-FUCOSYLTRANSFERASE 10 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGAGGAAGGGTTGATGCCTTCCTGAAATGAAATATGTGCCAGAGCCCG
Internal bar code:GGGGAAGGGATATCTGGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1119
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCATACGGTTGACACGTGCA
Suggested primer 2:ACCTTGGAAACGTTGGGTGT