| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042792 |
| Chromosome: | chromosome 6 |
| Location: | 8644221 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309951 | (1 of 1) 3.2.1.18 - Exo-alpha-sialidase / Sialidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCGGAGCCGCGGCTTCCTGGTGGGCCAGCTGCAGCGCTTCGGTGTGGA |
| Internal bar code: | AAGACTGATATGATTGCCCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAGTCATCGCCCCAGGAC |
| Suggested primer 2: | GACGCAGATCCTGACCTACG |