| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.042802 |
| Chromosome: | chromosome 16 |
| Location: | 2980620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663700 | (1 of 1) K03871 - von Hippel-Lindau disease tumor supressor (VHL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGGGGTCGGGGTCGCCACACGCAATGGATGACGTGTATGCAGATGAG |
| Internal bar code: | TGAGGCGATGGCATGCGCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTATGGACGCAAGCTGCT |
| Suggested primer 2: | GTGGACCGTTGACACGTTTG |