| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.042809 |
| Chromosome: | chromosome 12 |
| Location: | 3465461 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497350 | PPR13 | PentatricoPeptide Repeat protein 13 | CDS/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCACTCCGAGCTCGCGGTTCCCCGCGTGCCCCGACCCTTGCGCCCCGCC |
| Internal bar code: | GGTGATTCAGGTTCGAGATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1090 |
| LEAP-Seq percent confirming: | 48.5714 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGCTCTCCCTCCTTTGC |
| Suggested primer 2: | GGCGGAAATGCGTTGATACC |