Insertion junction: CLIP2.042811_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042811
Chromosome:chromosome 13
Location:3409481
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g586800 (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGACAGGTCAGCCTCCGGATGCAACTTCCGCCCTACCCCACCCTGGC
Internal bar code:AGTTTTCGCTAGTGTAGACTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:4961
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:ACACTCATCCTAGGCGAGGT
Suggested primer 2:ACGCGGGCTGTCAATAGAAA