Insertion junction: CLIP2.042817_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042817
Chromosome:chromosome 2
Location:6827540
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388319 (1 of 1) PTHR13390:SF0 - UPF0554 PROTEIN C2ORF43 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGCTGCGGCAGCGACCGCGTCGTCGTCAGTAGCAGTGCTCGCGAGCAG
Internal bar code:AAAGTTTGGCATAGTCAGGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2090
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCACTCGCTCACAATCG
Suggested primer 2:CCCTAGCGTGTCCTGCTATG