| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042819 |
| Chromosome: | chromosome 7 |
| Location: | 439819 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315250 | CSG5 | Chlamydomonas specific family G protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACGCGCTCCACCGCCTTCTGCTGCTCCACCGGCGTCGTGCCGCCCAAG |
| Internal bar code: | CATGGGAAACGTTCTCTTCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4028 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGTGATGTGGGTCGGAG |
| Suggested primer 2: | ACTCCCTAGCCACGATGACT |