| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.042821 |
| Chromosome: | chromosome 17 |
| Location: | 3438126 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723500 | (1 of 1) 3.6.3.30 - Fe(3+)-transporting ATPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTATGCAAGCATGCATGTACCGGTTTTCAGCTTTGAATGCGTCCACCC |
| Internal bar code: | TTTTAACCACAAGATAGCGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1226 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGTAGTCAGGGGAGCGG |
| Suggested primer 2: | TCGTGGTAACTGAAGTGGGC |