| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.042824 |
| Chromosome: | chromosome 3 |
| Location: | 4219735 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g173700 | KIL14 | Putative kinesin-like motor protein; (1 of 1) K10396 - kinesin family member 5 (KIF5) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGGCGGGGAGCGAGAGCATCTCGCACGCCAGCAGCAATGAGCAGCAG |
| Internal bar code: | TCCTGACGTTTAGTTAGGCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 57.1429 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTTATTGATGGCCTGCAG |
| Suggested primer 2: | GGAACACAACTGCTCGAGGA |