Insertion junction: CLIP2.042825_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042825
Chromosome:chromosome 1
Location:3351505
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g021700 CYG36 (1 of 5) IPR000104//IPR001054//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Adenylate/guanylate cyclase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTCTCGCTCTCGGCATGCAGCTCTACAGCCGCCTGGATGCCATGATT
Internal bar code:CAGGAAGTCTAGTTTAATCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2751
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CATCCACCCACCTTGACCTC
Suggested primer 2:GTAGGTGTAGGTGTGTGCCC