| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042834 |
| Chromosome: | chromosome 16 |
| Location: | 510177 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692800 | AKR4 | Aldo/keto reductase; (1 of 1) PTHR11732//PTHR11732:SF284 - ALDO/KETO REDUCTASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTCACCAGGGGTCGACTTGTACACGCCTGGGGCGTGGAATAGGGTGC |
| Internal bar code: | ATTCTATTTTTAGCTGATCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2776 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCACGTTCGTTTCTCGCA |
| Suggested primer 2: | CTTGCTGGTCACGTACACCT |