Insertion junction: CLIP2.042845_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042845
Chromosome:chromosome 6
Location:3715611
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278158 (1 of 2) IPR001283//IPR003882//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Pistil-specific extensin-like protein // CAP domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGCTGCCACCACGTCCACCACGCCCGGCGCACAGCCCTCGTGTGTTCA
Internal bar code:CTATACGGCGCGAAAGGTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:32.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCGTGCAGAAACCAACAT
Suggested primer 2:GCGACATTTGCACTTCAGCA