Insertion junction: CLIP2.042855_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042855
Chromosome:chromosome 17
Location:2750239
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g717800 NUP93 Nucleoporin 93; (1 of 1) K14309 - nuclear pore complex protein Nup93 (NUP93, NIC96) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGATGGATGGACGGTTGTTGTTGTAGACTTGTGTTGCCCCGTTTGAAAG
Internal bar code:GACGTAACGCAATATAACCATG

Confirmation - LEAP-Seq

LEAP-Seq distance:677
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTGCTTTCCCGATCTGTG
Suggested primer 2:CCTCACCCACACCCACATAC