| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042864 |
| Chromosome: | chromosome 9 |
| Location: | 3238450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394769 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTGACGTGGCTTGCGGGCGGCGGCGGCGGCGCGGTGAGCGAGTGGT |
| Internal bar code: | AGGGATAGTTAGTCCTCGCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1504 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCTGCACAAGTGAGCCAG |
| Suggested primer 2: | GATCGAGGCAAGGAGGATGG |