Insertion junction: CLIP2.042869_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.042869
Chromosome:chromosome 15
Location:347225
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801684 (1 of 1) PF12051 - Protein of unknown function (DUF3533) (DUF3533) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGGCGTGCGCGGCTTCCGCCACATCTTCTTCGGCGCCCTCAACGACACC
Internal bar code:ATTGATCCGCATGTATTGCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:89
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCTCACAAACACGTCAGC
Suggested primer 2:GGCCGTGGCTAGTTGTGTAT