| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042880 |
| Chromosome: | chromosome 16 |
| Location: | 6348134 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675700 | BLZ21 | (1 of 2) IPR000104//IPR004827 - Antifreeze protein, type I // Basic-leucine zipper domain; bZIP transcription factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACACACCCTCGAGAACAAGAACCGTGGTTCCGCTGCCGGGTGTCGT |
| Internal bar code: | TTAATTTCGGAGTACGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 47.0588 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACTTGCTGCACACACAC |
| Suggested primer 2: | CGCTTGCTTGTCGTCAAACA |