| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042897 |
| Chromosome: | chromosome 3 |
| Location: | 8129257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200800 | CYG61 | (1 of 11) 4.6.1.1//4.6.1.2 - Adenylate cyclase / ATP pyrophosphate-lyase // Guanylate cyclase / Guanylyl cyclase; Adenylate/guanylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATGAAGGGCGCCAGAGTCCGCTGCGCTTCCGCCGGGTCCCAGCCCG |
| Internal bar code: | GACTTGAGAACGATTAACCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2170 |
| LEAP-Seq percent confirming: | 59.5238 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGAGCTTTGGTGGAGAGT |
| Suggested primer 2: | CAACTTACTTGCACCGTGGC |