| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042902 |
| Chromosome: | chromosome 1 |
| Location: | 5482536 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g038800 | MIP3 | (1 of 1) K09875 - aquaporin SIP (SIP); Major intrinsic protein superfamily | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCGCGTTGCGCTTTGGCCCAGCTGCACGCCACCACGCACGCACCACAT |
| Internal bar code: | GTTAAAGGAGCAAGGTGCTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4791 |
| LEAP-Seq percent confirming: | 97.037 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 135 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTATTCGGTGGCCCTA |
| Suggested primer 2: | ACCAAGTTGCTGACACCACA |