| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042904 |
| Chromosome: | chromosome 6 |
| Location: | 4766603 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g279650 | OPR24 | OctotricoPeptide Repeat protein 24 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGGAGGTCAGTGTGGGGAGGCACTTTGGGATGATTCGCTGTCCATAGC |
| Internal bar code: | GGAACGTCAGAAACGATGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4970 |
| LEAP-Seq percent confirming: | 66.1765 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATGCTGCAATAGAACGC |
| Suggested primer 2: | GCACACTTCACCGGTTAGGA |