| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042910 |
| Chromosome: | chromosome 10 |
| Location: | 1722616 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430100 | ELG22 | Exostosin-like glycosyltransferase 22; (1 of 6) IPR000742//IPR004263//IPR009030 - EGF-like domain // Exostosin-like // Insulin-like growth factor binding protein, N-terminal | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACATGTATTACTCGAGCACCTTCGGGGAGATCATGGGCGAAGACGGCC |
| Internal bar code: | GACCTGGTAATTGTTCCCAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5142 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTCCCCCGAGAAAGAGG |
| Suggested primer 2: | ATGACCGCGTACACTTTGGT |