| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.042926 |
| Chromosome: | chromosome 17 |
| Location: | 6495164 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g744497 | (1 of 1) PF09783 - Vacuolar import and degradation protein (Vac_ImportDeg) | CDS | |
| lncRNA_TCONS_00240303 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGAGGAGGTCCGGCCGGTTCCCACTGACGTGCCGTCGGTGCCTTCCT |
| Internal bar code: | TTAGAAAATCCGTGAAACATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAACGGGAAACACACAGC |
| Suggested primer 2: | TACTTCTTCTACCGCGGGGA |