| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042927 |
| Chromosome: | chromosome 3 |
| Location: | 5606698 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185750 | (1 of 1) IPR003613//IPR013083 - U box domain // Zinc finger, RING/FYVE/PHD-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGGCAGGAACTCCAGCTCTGCAGTCCAGACCCCGAAGAGGGGTATGA |
| Internal bar code: | GAGCTTTCTGTGATCGAGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3303 |
| LEAP-Seq percent confirming: | 84.375 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTCCTTTCCAGCTGAG |
| Suggested primer 2: | GCAAATGTCCGCACTCACTG |