Insertion junction: CLIP2.042933_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042933
Chromosome:chromosome 6
Location:2801435
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g271700 (1 of 2) 3.4.17.4 - Gly-Xaa carboxypeptidase / Glycine carboxypeptidase; Metallopeptidase M20 family protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCAGATTCCCCGGTGCCGCAACGCTTTGTACGGCGCACTGTCAGAGT
Internal bar code:AGCATCATTTTACGTTTCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3398
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:83
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:83

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCAGCAACAAGGACAC
Suggested primer 2:GTGCTTGTTCCATTGCAGCA