| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.042975 |
| Chromosome: | chromosome 17 |
| Location: | 1709633 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708300 | RPN12 | (1 of 1) K03031 - 26S proteasome regulatory subunit N12 (PSMD8, RPN12); 26S proteasome regulatory subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGATCCGGTATCGGGCCTTTCGACGCTGGTCGGATGGTGGTAGGTTG |
| Internal bar code: | AAACAATTGAGATAGTTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1222 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGTCTTCGACGCCCAT |
| Suggested primer 2: | AGTTACGTGTCCGGTGCATT |