Insertion junction: CLIP2.042977_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.042977
Chromosome:chromosome 10
Location:5255163
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g456350 KIN7-2,KIN7B Kinesin motor protein; (1 of 1) PF00225//PF11995 - Kinesin motor domain (Kinesin) // Domain of unknown function (DUF3490) (DUF3490) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACCTGCGCAGCGAGGTCGCGCGCCTGCAGCGCATGCGGGTGCGTTTGT
Internal bar code:GTAAACGAGAGGCAATGAATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1601
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGCCGTACAACCCATCTT
Suggested primer 2:ACCGAGATTGGAAAGCTGCA