| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.042982 |
| Chromosome: | chromosome 2 |
| Location: | 4099815 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g103400 | OPR118 | OctotricoPeptide Repeat protein 118 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGTCCTTGTACTCCGCCATCCAGTCGCCGCCTGGTGGACAGGTATGTA |
| Internal bar code: | TCACCTCAGGGCTACTGAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 17.6471 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAATCTGGACCTGGAGGCG |
| Suggested primer 2: | CTTGAGAGCGTGTGGACACT |