| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.042988 |
| Chromosome: | chromosome 14 |
| Location: | 1444489 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617250 | FAP282 | Coiled-Coil Flagellar Associated Protein 282 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCAAAGTTCCAGGTGTACGTCCGGGCATAGATACGCCTTACTCCTCA |
| Internal bar code: | GGTAAAGTGGAGTATGAAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4164 |
| LEAP-Seq percent confirming: | 95.1923 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 104 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCAACCATTAGATGCAGC |
| Suggested primer 2: | ACATGCAAGAGGTGACAGGG |