Insertion junction: CLIP2.043010_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.043010
Chromosome:chromosome 12
Location:7616646
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555800 STK2,STPK2 Serine/threonine protein kinase; (1 of 1) PTHR24362//PTHR24362:SF286 - SERINE/THREONINE-PROTEIN KINASE NEK // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTAGGAACGCGGGCCGGGAACAACCACACTGCAACATGGTCCCTGCCATC
Internal bar code:TTGGAAGGCCGTTAGCGGATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1835
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCCGCTCTGTAACCAACCTT
Suggested primer 2:AACTCTCCTCCTCCTCCGTC