| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043020 |
| Chromosome: | chromosome 13 |
| Location: | 2387839 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g579250 | (1 of 41) PTHR32523//PTHR32523:SF8 - FAMILY NOT NAMED // PHYTOL KINASE 1, CHLOROPLASTIC | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCGTGGCACTACTGTCCTGCGCAACCACCCACTCCAGCACCAAGTTG |
| Internal bar code: | GTACCGTGTAGCATTGGTGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3132 |
| LEAP-Seq percent confirming: | 2.22222 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCATGTCGTGAACTGCG |
| Suggested primer 2: | GTGCTGCAACATTAGCTCGG |