Insertion junction: CLIP2.043036_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.043036
Chromosome:chromosome 6
Location:6490177
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g294050 (1 of 2) PTHR22640:SF2 - STRUCTURAL MAINTENANCE OF CHROMOSOMES FLEXIBLE HINGE DOMAIN-CONTAINING PROTEIN 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGGATGCAGGAACTCACCTTCACAGGGCGTGCCCTACTACTGAGTCG
Internal bar code:GCTCGTTTAGACTACGGCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3456
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:TGGTTCTCCTTTCCGCCTTC
Suggested primer 2:AACTGGGAGAAGCCATGACG