Insertion junction: CLIP2.043047_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043047
Chromosome:chromosome 7
Location:422825
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315050 GGH1 (1 of 2) 3.4.19.9 - Gamma-glutamyl hydrolase / Pteroyl-poly-alpha-glutamate hydrolase; Gamma-glutamyl hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAAGTTAAGCTACCGGCGCACCGGCCACGAATAGGCGACCACAAAGGC
Internal bar code:AGCTCATTGTCGCAATTTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:430
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CAATCCCACGCCTATCCTCC
Suggested primer 2:CCTCCCTCCCCTTCTCATGA