Insertion junction: CLIP2.043050_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.043050
Chromosome:chromosome 13
Location:2138754
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g577550 DDB2 (1 of 1) K10140 - DNA damage-binding protein 2 (DDB2); UV-damaged DNA binding complex subunit 2 protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTCTTACGTAATCATGTTTGTAACGCGCCCGTCACCGCACCCCCGTC
Internal bar code:TGAAGTATCATACGTCGAGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:59
LEAP-Seq percent confirming:28.5714
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTCCCATTGTGTCCAGTC
Suggested primer 2:TCGGTCCATCGCATACACTG