| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.043055 |
| Chromosome: | chromosome 6 |
| Location: | 3835865 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278178 | (1 of 1) PF09495 - Protein of unknown function (DUF2462) (DUF2462) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGAATGGCCATCCTAGCAGCTACCACACTGGCAGCCGCGTCGGTTGA |
| Internal bar code: | ATGACCATGTGATACACAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2431 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCATCACATCGACACACA |
| Suggested primer 2: | CGTCCTGGTAGGGCTTGTTT |