Insertion junction: CLIP2.043056_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.043056
Chromosome:chromosome 16
Location:7338551
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g679221 ANK27 Predicted protein with ankyrin repeats; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTGGTGGCGGCCGGAGCCAACATGAACTACCCGGGGAAGGTGCGCTTG
Internal bar code:AGAGGGCTTCAGTGCCACCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:7292
LEAP-Seq percent confirming:86.1538
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:ATCACCCGCACTGTGAATGT
Suggested primer 2:CAAGTTTGCATGGTTCCCGG