| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.043058 |
| Chromosome: | chromosome 11 |
| Location: | 2046691 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467759 | PIC1,PIC1-2 | (1 of 2) PTHR34548:SF2 - PROTEIN TIC 21, CHLOROPLASTIC; Similar to AtTic21/PIC1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAACGTGGCGGCCACGGTGGGTGATGGGTGATGGCCTGGTGGGTGGGT |
| Internal bar code: | TTTTTGGCTGCCTGGGGCAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 5.88235 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAAGAAGGTGGACAGGAA |
| Suggested primer 2: | CCATCACAATGCAGCTACGC |